Comparative genomics reveals expansion of the FLC region in the genus Arabidopsis |
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Authors: | Abhijit Sanyal Scott A Jackson |
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Institution: | (1) Purdue University, 915 West State St., West Lafayette, IN 47907, USA |
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Abstract: | Mechanisms of genome evolution are poorly understood although recent genome sequencing is providing the tools to begin to
illuminate such mechanisms. Using high-resolution molecular cytogenetic tools, we examined the structural evolution of 790 kb
surrounding the evolutionarily important FLC locus of Arabidopsis thaliana in three of its relatives, Arabidopsis halleri, Arabidopsis neglecta and Arabidopsis arenosa. Sequenced BACs from A. thaliana were used as heterologous probes across these species and genome expansion was found in all three species relative to A. thaliana, ranging from 16 to 27%. Expansion was seen along the length of the entire region but molecular analyses revealed no characteristic
pattern of either intra- or intergenic expansion among these species. Mapping of BACs on DNA fibers from A. thaliana revealed one possible error, ~14 kb missing from the reported sequence, indicating that for comparative studies it is important
to confirm the reference sequence to which comparison will be made. |
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Keywords: | Arabidopsis Comparative genomics FISH Physical mapping Cytogenetics |
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