Ultrastructural analysis of leaf trichome plasmodesmata reveals major differences from mesophyll plasmodesmata |
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Authors: | Elisabeth Waigmann Adrian Turner Jan Peart Keith Roberts Patricia Zambryski |
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Institution: | (1) Department of Plant Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA, US;(2) Department of Cell Biology, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK, GB |
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Abstract: | Functional studies on molecular transport through plasmodesmata in leaf mesophyll and trichome cells revealed significant
differences in their basal size-exclusion limits and their response to microinjected tobacco mosaic virus movement protein
(E. Waigmann et al., 1994, Proc. Natl. Acad. Sci. USA 91: 1433–1437; E. Waigmann and P. Zambryski, 1995, Plant Cell 7: 2069–2079).
To address the basis for these functional differences, Nicotiana clevelandii trichome and mesophyll plasmodesmata were compared ultrastructurally. Trichome plasmodesmata increase in ultrastructural complexity
from the tip to the base cell. Their neck regions, thought to control molecular traffic through plasmodesmata, are clearly
distinct from necks of mesophyll plasmodesmata. In contrast to the electron-dense desmotubular area in mesophyll plasmodesmata,
trichome plasmodesmata contain an electron-translucent circle in their center, surrounded by an electron-dense ring. This
latter ring is connected to the inner leaflet of the plasma membrane by multiple spokes or filaments. Two monoclonal antibodies
raised against a maize plasmodesmal protein preparation (A. Turner et al., 1994, J Cell Sci. 107: 3351–3361) interact with
both trichome and mesophyll N. clevelandii plasmodesmata. Based on the localization pattern and the high degree of cross-reactivity, both antibodies likely recognize
a conserved structural component of plasmodesmata, and may be useful to mark plasmodesma in a variety of plants and tissues.
Received: 24 January 1997 / Accepted: 3 March 1997 |
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Keywords: | : Antibody (Plasmodesma) Nicotiana (plasmodesmata) Plasmodesmal (antibody ultrastucture) Trichomes Ultrastructure (plasmodesmata) |
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