nm23、EGFR和Bcl-2蛋白在肺癌组织中的表达及临床意义

目的:观察nm23、EGFR和Bcl-2在人肺癌中的表达,探讨其与肺癌临床生物学行为的关系。方法:采用免疫组化检测了49例肺癌及癌旁组织中nm23、EGFR和Bcl-2蛋白的表达情况,并对其与肺癌临床生物学行为进行相关分析。结果:在49例肺癌中,EGFR和Bel-2蛋白的阳性表达率分别为51.0%和34.7%,均高于正常肺组织17.5%和12.5%(p<0.05),nm23蛋白的阳性表达率为55.1%,则显著低于正常肺组织87.5%(p<0.01)。在病理分级的高一中分化、低分化和未分化中nm23的阳性表达率分别为82.6%、41.2%和11.1%(p<0.01)。EGFR在非小细胞肺癌(NSCLC)组为61.5%,在小细胞肺癌(SCLC)组为10.0%(p<0.01)。在NSCLC TNM分期中,Ⅰ期 Ⅱ期与Ⅲ P<0.01)期中EGFR的阳性表达率分别为39.3%和81.8%(p<0.01);病理分级的高-中分化和低分化中EGFR的阳性表达率分别为39.1%和88.2%(p<0.01);在淋巴结转移组为57.9%,无淋巴结转移组为27.3%(p<0.05)。Bcl-2的阳性表达率在NSCLC组为25.6%,在SCLC组为70%,统计学分析差异有统计学意义(p<0.01);在NSCLC组病理分级的高分化、中分化和低分化中Bcl-2阳性表达率分别为62.5%、26.7%和5.89%(p<0.01)。结论:癌基因EGFR、凋亡抑制基因Bcl-2与抑癌基因nm23在肺癌的发生、发展中存在着相互促进作用。

Study on Expression of nm23, EGFR and Bcl-2 in Lung Neoplasms and their Clinical Significance

Objective:To investigate the relationship between non-metastatic gene(nm23),epidermal growth factor receptor (EGFR) and B celllymphoma/leukemia-2(Bcl-2)and the clinical and biological features of human lung cancer by observing the expres- sion of nm23,EGFR and Bcl-2 protein in lung cancer.Methods:Paraffin embedded specimens of 49 lung cancer tissues and non-tumor lung tissues were selected,in which,the expression of nm23,EGFR and Bcl-2 was detected by immunohistochemical method.Correla- tion was studied by statistical analysis of clinical biological behaviors of lung cancer.Results:In 49 lung cancer tissues,the positive ex- pression rates of EGFR and Bcl-2 were 51.0% and 34.7% respectively,which were significantly higher than that of non-tumor lung spec- imens(17.5%,12.5%)(p<0.05).The positive expression rates of nm23 was 55.1%,which was much lower than that of non-tumor lung specimens(87.5%)(P<0.01).Significant differences of the expression rate of EGFR were found between non-small cell lung cancer (NSCLC)and small cell lung cancer(SCLC)(p<0.01).This result indicated that EGFR might be related to the generation and develop- ment of NSCLC.The expression of EGFR was markedly correlated to TNM,pathological grade and metastasis of human NSCLC(p<0. 05).It showed the high expression of EGFR found in those tumors that were more malignant and poorer differentiated and later stage. The expression level of Bcl-2 in SCLC was significantly higher than NSCLC (p<0.01),and it suggested that the high expression of Bcl-2 had the fight correlation with the biological characters of SCLC.In NSCLC,the Bcl-2 level was associated with clinco-pathologieal grade (p<0.01),and Bcl-2 was supposed to be involved in the oncogenesis of early-stage lung cancer.In lung cancer tissues,there was obvious negative correlation between nm23 and EGFR as well as EGFR and Bcl-2(r=-0.488,p<0.01;r=-0.551,p<0.01 ).Conclusion:In the oncogenesis and development of lung cancer,there was mutual promotion between nm23,EGFR and Bcl-2.