食管鳞状细胞癌癌组织中hMLH1、hMSH2的表达及其意义

目的:探讨错配修复基因hMSH2、hMLH1在食管鳞癌组织中的表达及其临床意义。方法:采用sP免疫组化的方法检测144例食管鳞癌手术切除标本、30例正常粘膜组织中hMSH2、hMLH1表达情况,并与临床病理参数作相关分析。结果:食管鳞癌组织hMSH2、hMLH1基因阳性表达明显下降,与正常食道粘膜相比相差显著(P<0.01),hMSH2、hMLH1表达缺失与年龄、性别、肿瘤大小、淋巴结转移、浸润深度无显著相关,与肿瘤的分化程度有关(P<0.01)。结论:提示hMLH1和hMSH2基因突变所致的蛋白表达异常缺失与食管鳞状细胞癌发生有关,可能为一种早期事件。

Expression and Clinical Significance ofhMLH1, hMSH2 in Esophagus Squamous Cancer

Objective:To investigate the expression of mismatch repair gene hMLH1 and hMSH2 in esophageal squamous cancer and its clinical significance.Methods:We detected the expression of hMLH1 and hMSH2 respectively in esophageal carcinoma from 144 patients and 30 normal mucosa tissues by SP immunohistochemistry and correlation analysis with clinical patho-parameters.Results:The positive rate of hMLH1 and hMSH2 expression in esophageal squamous cancer tissues was significantly lower than that in normal mu- cosa tissues.The expression of hMLH1 and hMSH2 protein was closely related to differentiation degree of the cancer,but not obviously to age,gender,lymph node metastasis,tumor size and depth of invasion.Conclusions:It shows that the disfigurement of hMLH1 and hMSH2 expression resulted from gene mutation may be related to esophageal squamous carcinogenesis,and it may be an earlyevent.