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PCR-Based Identification of Hyperthermophilic Archaea of the Family Thermococcaceae |
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| Authors: | Galina B. Slobodkina Nikolai A. Chernyh Alexander I. Slobodkin Irina V. Subbotina Elizaveta A. Bonch-Osmolovskaya Alexander V. Lebedinsky |
| Abstract: | A method for rapid detection and identification of hyperthermophilic archaea of the family Thermococcaceae based on PCR amplification of 16S rRNA gene fragments with primers TcPc 173F (5′-TCCCCCATAGGYCTGRGGTACTGGAAGGTC-3′) and TcPc 589R (5′-GCCGTGRGATTTCGCCAGGGACTTACGGGC-3′) was developed and used for identification of new isolates. |
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