Increased DNA topoisomerase I activity in aging human cell chromatin |
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Authors: | Claude Hamelin Lise Cousineau Michel Dion Jocelyn Yelle |
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Affiliation: | (1) Centre de recherche en virologie, Institut Armand-Frappier, C.P. 100, H7N 4Z3 Laval-des-Rapides, Québec, Canada |
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Abstract: | Chromatin-associated DNA topoisomerase I activity was measured in human diploid fibroblasts during in vitro aging. No diilerence was detected as a function of cell age in the nicking and the closing activities of the DNA-unwinding enzyme. The capacity of type-I topoisomerase to relax superhelical DNA molecules was, however, increased in aged cells. An age-related increase in nucleoprotein content was also observed. |
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