Localization of peanut (Arachis hypogaea) root lectin (PRA II) on root surface and its biological significance |
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| Authors: | Gurpreet Kalsi Cherukuri R Babu and Rakha H Das |
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| Institution: | (1) C.S.I.R. Centre for Biochemical Technology, Mall Road, Delhi University Campus, 110007 Delhi, India;(2) Department of Botany, Delhi University, 110007 Delhi, India |
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| Abstract: | The glucose-specific peanut root lectin, PRA II, is localized on the surface of 7-day-old peanut seedling root and in root cortical parenchymatous cells. The lectin is eluted from intact roots upon washing with buffer containing glucose. Rabbit erythrocytes bind to the root surface and the cortical cells; the binding is inhibited by antibodies raised against PRA II, peanut-specificRhizobium cells and by glucose. Lipopolysaccharides isolated from host-specificRhizobium strain inhibit the haemagglutinating activity of PRA II and are precipitated by the lectin. Our results suggest that PRA II might be involved in recognition ofRhizobium by peanut roots. |
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| Keywords: | root lectin peanut lipopolysaccharides symbiosis Rhizobium |
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