Preservation of Soluble Proteins for Histological Staining in Paraffin Sections |
| |
Authors: | John M. Craig Charles D. Cook Jere Mead |
| |
Affiliation: | a The Children's Cancer Research Foundation and the Department of Pathology, The Children's Medical Center and Harvard Medical School, Boston, Massachusettsb Department of Pediatrics, The Children's Medical Center and Harvard Medical School, Boston, Massachusettsc Harvard School of Public Health, Boston, Massachusetts |
| |
Abstract: | Human serum at full strength and in dilutions with physiological saline (0.85%) ranging from 1:1 to 1:72 was allowed to permeate rectangular masses of fibrin foam in small pieces (maximum diameters 0.2 × 0.4 × 1.0 cm), and then placed in 10% neutral formalin, Zenker's solution and Bouin's solution. After fixation for 4-12 hr, the fibrin foam and occluded serum proteins were imbedded, sections cut and stained with eosin bluish (CI. 771), 0.25% alcoholic solution, and by the McManus periodic acid-Schiff technique, using basic fuchsin (CI. 677). Undiluted serum (6.4 gin 100 ml) was not stainable after fixation in 10% formalin. With Zenker's solution stainable serum proteins are recognizable at 0.22 gm/100 ml and with Bouin's solution at 0.08 gm/100 ml. Dried aliquots (0.2 ml) of the same dilutions, spread over an area of 1.0 cm2, fixed and stained similarly, gave almost identical results. |
| |
Keywords: | |
本文献已被 InformaWorld 等数据库收录! |
|