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RPPAML/RIMS: A metadata format and an information management system for reverse phase protein arrays |
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| Authors: | Romesh Stanislaus Mark Carey Helena F Deus Kevin Coombes Bryan T Hennessy Gordon B Mills Jonas S Almeida |
| Institution: | 1.Department of Bioinformatics and Computational Biology,The University of Texas M. D. Anderson Cancer Center,Houston,USA;2.Department of Systems Biology,The University of Texas M. D. Anderson Cancer Center,Houston,USA;3.Instituto de Tecnologia Química e Biológica,Universidade Nova de Lisboa,Lisbon,Portugal |
| Abstract: | BackgroundReverse Phase Protein Arrays (RPPA) are convenient assay platforms to investigate the presence of biomarkers in tissue lysates. As with other high-throughput technologies, substantial amounts of analytical data are generated. Over 1000 samples may be printed on a single nitrocellulose slide. Up to 100 different proteins may be assessed using immunoperoxidase or immunoflorescence techniques in order to determine relative amounts of protein expression in the samples of interest. |
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