Synthesis of salt-soluble proteins in barley. Pulse-labeling study of grain filling in liquid-cultured detached spikes |
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Authors: | Henriette Giese Jørn Hejgaard |
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Institution: | (1) Agricultural Research Department, Risø National Laboratory, DK-4000 Roskilde, Denmark;(2) Department of Biochemistry and Nutrition, Technical University of Denmark, DK-2800 Lyngby, Denmark |
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Abstract: | The accumulation of salt-soluble proteins in the endosperm of developing barley (Hordeum vulgare L.) grains was examined. Detached spikes of barley were cultured at different levels of nitrogen nutrition and pulse-labeled with 14C] sucrose at specific times after anthesis. Proteins were extracted from isolated endosperms and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and crossed immunoelectrophoresis. Fluorography revealed an early, middle and late synthesis of specific proteins during grain filling. Synthesis of proteins appearing at the later stages responded to increased nitrogen nutrition. Two major components, -amylase and protein Z in particular, had a synthesis profile almost identical to that of the endosperm storage protein, hordein.Abbreviations CIE
Crossed immunoelectrophoresis
- SDSPAGE
Sodium dodecyl sulphate polyacrylamide gel electrophoresis |
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Keywords: | Grain filling Hordeum (storage protein) Lysine rich protein Spike culture Protein (salt-soluble) Storage protein |
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