Genetic diversity assessment of summer squash landraces using molecular markers |
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Authors: | Emad A. Mady Alaa Al-Din Helaly Abdel Naem Abu El-Hamd Arafa Abdou Shamel A. Shanan Lyle E. Craker |
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Affiliation: | 1. Medicinal Plant Program, The Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA, USA 2. Horticulture Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
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Abstract: | Plant identification, classification, and genotyping within a germplasm collection are essential elements for establishing a breeding program that enhances the probability of plants with desirable characteristics in the market place. In this study, random amplified polymorphic DNA (RAPD) was used as a molecular tool to assess the diversity and relationship among 20 summer squash (Curcubita pepo L.) landraces traditionally used to treat hypertension and prostate hyperplasia. A total of 10 RAPD primers produced 65 reproducible bands of which 46 (70.77 %) were polymorphic, indicating a large number of genotypes within the summer squash lines. Cluster analysis divided the summer squash germplasm into two groups, one including one landrace and a second containing 19 landraces that could be divided into five sub-groups. Results of this study indicate the potential of RAPD markers for the identification and assessment of genetic variations among squash landraces and provide a number of choices for developing a successful breeding program to improve summer squash. |
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