Occurrence of selenocysteine in the selenium-dependent formate dehydrogenase of Methanococcus vannielii. |
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Authors: | J B Jones G L Dilworth T C Stadtman |
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Institution: | Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20014 U.S.A. |
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Abstract: | The selenium-dependent formate dehydrogenase of Methanococcus vannielii was isolated from bacteria grown in the presence of ]selenite. Purification under strictly anaerobic conditions resulted in the simultaneous enrichment of formate dehydrogenase activity, , and a brown chromophore that absorbs maximally at 380 nm. Acid hydrolysis of the enzyme after reduction with borohydride and alkylation with iodoacetamide, released a radioactive selenoamino acid derivative that was identified as ]carboxymethyl-selenocysteine. This is the third selenoenzyme shown to contain selenocysteine. |
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Keywords: | To whom all correspondence should be sent |
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