大鼠液压冲击脑损伤脑干c—jun mRNA表达的定位观察

目的：研究大鼠中度侧位液压冲击脑损伤时脑干c-jun mRNA及其表达产物Jun变化规律。方法：雄性SD大鼠,随机分为正常对照组、手术对照组和损伤组。损伤组动物均给以0.2MPa液压冲击脑损伤,按冲击后处死时间不同再分为5min、15min、30min、1h、2h、4h、8h和12h组。应用免疫组织化学和原位杂交方法观察c-jun在脑干的表达。结果：脑冲击后15min-12h,Jun阳性细胞数逐渐增多。冲击后5min,c-jun mRNA表达开始增强,2h表达最强,然后逐渐减弱。结论：侧位液压冲击脑损伤后c-jun在脑干表达迅速增强,持续时间较长。

Expression of c-jun mRNA in brain stem following moderate lateral fluid percussion brain injury in rats

Aim: To study the expression of c jun in brain stem following moderate lateral fluid percussion brain injury in rats,and to observe the temporal patterns of its expressions following percussion. Methods: Male Sprague Dawley rats were divided into normal control,sham operation control and injury groups. The rats of injury group subjected to moderate lateral fluid percussion injury (0.2MPa),and then were subdivided into 5 min,15 min,30 min,1 h,2 h,4 h,8 h and 12 h groups according to the time elapsed after injury. The expression of c jun was studied by immunohistochemistry and in situ hybridization. Results: After percussion for 15 min,Jun positive neurons increased in brain stem progressively. At 5 min after percussion,the induction of c jun mRNA was increased,remained elevated up to 2h after brain injury and then decreased gradually. Conclusion: The induction and expression of the c jun in brain stem after fluid percussion brain injury were increased rapidly and lasted for a long time.