Escherichia coli parA is an allele of the gyrB gene |
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Authors: | Jun-ichi Kato Yukinobu Nishimura and Hideho Suzuki |
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Institution: | (1) Laboratory of Genetics, Department of Biology, Faculty of Science, University of Tokyo, Hongo, 113 Tokyo, Japan;(2) National Institute of Genetics, 411 Mishima, Japan;(3) Present address: Department of Bacteriology, National Institute of Health, 141 Tokyo, Japan |
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Abstract: | Summary A thermosensitive (ts) parA mutant, MFT110, of Escherichia coli carried at least two ts mutations. The major ts defect, resulting from a mutation mapped originally at 95 min and complemented by pLC8-47, was most probably due to psd. A plasmid carrying the 1.6 kb BamHI-PvuII fragment recloned from pLC8-47 complemented the major ts mutation in MFT110 and psd(ts) in two mutants, but did not correct the Par phenotype of MFT110. The second ts mutation was salt-repairable and mapped at 83 min close to recF and tnaA. This mutation was linked with the Par phenotype as shown unambiguously by 4 ,6-diamidino-2-phenylindole stained nucleoids in parA mutant cells with the W3110 genetic background. Both salt-repairable ts and Par traits were corrected concomitantly by a plasmid carrying the chromosomal region solely for the gyrB gene. This strongly suggests that parA is an allele of gyrB. |
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Keywords: | Chromosome partition parA gyrB psd |
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