Detection of Campylobacter jejuni in food and poultry viscera using immunomagnetic separation and microtitre hybridization |
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| Authors: | M Lamoureux A MacKay S Messier I Fliss BW Blais RA Holley & RE Simard |
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| Institution: | Facultéde médecine vétérinaire, Universitéde Montréal, St-Hyacinthe, Québec,;Centre de recherche en science et technologie du lait (STELA), Département de Sciences et Technologie des Aliments, UniversitéLaval, Québec,;Laboratory Services Division, Food Production and Inspection Branch, Agriculture and Agri-Food Canada, Ottawa, Ontario,;Department of Food Science, University of Manitoba, Winnipeg, Manitoba, Canada |
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| Abstract: | Thermophillic Campylobacter and Camp. jejuni were detected from samplesof chicken liver, gall bladder, muscle and contaminated milk and chicken meat after anenrichment step by using immunomagnetic capture of cells with monoclonal antibody againsta specific outer membrane protein of thermophilic Campylobacter. The detection ofcaptured cells was achieved using two different hybridization methods. In one of the methods,the captured cells were lysed by guanidine isothiocyanate and the 23S rRNA wasreacted with a microtitre plate-immobilized rDNA probe specific for thermophilic Campylobacter. In the other method, the captured cells were subjected to lysis byultrasonication and the genomic DNA reacted with a microtitre plate-immobilized RNAprobe specific for Camp. jejuni. Detection of the RNA–DNA hybrids formed in the wells was carried out using a monoclonal anti-RNA–DNA hybrid antibody. |
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