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The role of transcortin in glucocorticoid mediated enzyme induction: tyrosine aminotransferase induction in hepatoma tissue culture cells
Authors:M Lippman  E B Thompson
Affiliation:1. Department of Internal Medicine, Yale Medical School, New Haven, CT 06510, U.S.A.;2. Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20014, U.S.A.;1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China;2. Children''s Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;3. Department of Oncological Sciences, University of Utah, Huntsman Cancer Institute, Salt Lake City, UT 84112, USA;4. Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;5. Department of Clinical Science, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;6. Department of Pediatrics and Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;7. School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China;1. Neurology Service, San Francisco Veterans Affairs Health Care System, San Francisco, CA 94121, USA;2. Dept. of Neurology, University of California San Francisco, San Francisco, CA 94143, USA;1. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China;2. Institute for Energy Research, Jiangsu University, Zhenjiang 212013, China;1. Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195-2120, USA;2. Condensed Matter Science and Technology Institute, School of Science, Harbin Institute of Technology, Harbin 150080, China;3. Department of Chemistry, University of Washington, Seattle, WA 98195-2120, USA
Abstract:Transcortin from rats selectively binds cortisol but not the synthetic glucocorticoid dexamethasone. Both steroids are potent inducers of the enzyme tyrosine aminotransferase in hepatoma tissue culture cells invitro under serum free conditions. Transcortin is shown to block selectively enzyme induction by cortisol but not dexamethasone. The inhibitory effect of added transcortin can be abolished by adding sufficient cortisol to overwhelm the transcortin binding capacity. It is also shown that transcortin can inhibit the entry of radiolabelled cortisol, but not dexamethasone, into hepatoma cells invitro. This probably is the mechanism which inhibits enzyme induction by cortisol. In addition a rapid and reproducible technique for estimating transcortin binding capacity is described.
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