SUPPRESSION OF AcMNPV REPLICATION BY ADF AND THYMOSIN PROTEIN UP‐REGULATION IN A NEW TESTIS CELL LINE,Ha‐shl‐t |
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| Authors: | Xiaoqian Zhang Ming Chen Xinlei Ma Xiaofan Zhao Jinxing Wang Honglian Shao Qisheng Song David Stanley |
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| Affiliation: | 1. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, , Jinan, China;2. Division of Plant Sciences, University of Missouri, , Columbia, Missouri, USA;3. Division of Plant Sciences, University of Missouri, Columbia, Missouri, USA and U.S. Department of Agriculture, Agricultural Research Service, Biological Control of Insects Research Laboratory, , Columbia, Missouri, USA |
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| Abstract: | Host cytoskeletons facilitate the entry, replication, and egress of viruses because cytoskeletons are essential for viral survival. One mechanism of resisting viral infections involves regulating cytoskeletal polymerization/depolymerization. However, the molecular mechanisms of regulating these changes in cytoskeleton to suppress viral replication remain unclear. We established a cell line (named Ha‐shl‐t) from the pupal testis of Helicoverpa armigera (Lepidoptera: Noctuidae). The new testis cell line suppresses Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV) replication via disassembly of cytoskeleton. Up‐regulation of thymosin (actin disassembling factor) and adf (actin depolymerizing factor) reduces F‐actin. Silencing thymosin or adf or treating cells with the F‐actin stabilizer phalloidin led to increased AcMNPV replication, while treating cells with an F‐actin assembly inhibitor cytochalasin B decreased viral replication. We infer that Ha‐shl‐t cells utilize F‐actin depolymerization to suppress AcMNPV replication by up‐regulating thymosin and adf. We propose Ha‐shl‐t as a model system for investigating cytoskeletal regulation in antiviral action and testicular biology generally. |
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| Keywords: | AcMNPV thymosin adf F‐actin testis cell line |
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