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Highly efficient production of the Alzheimer's γ‐Secretase integral membrane protease complex by a multi‐gene stable integration approach |
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| Authors: | Jean‐René Alattia Mattia Matasci Mitko Dimitrov Lorène Aeschbach Sowmya Balasubramanian David L. Hacker Florian M. Wurm Patrick C. Fraering |
| Affiliation: | 1. Laboratory of Molecular & Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland;2. telephone: 41‐21‐693‐96‐51, fax: 41‐21‐693‐95‐72;3. Laboratory for Cellular Biotechnology, Institute of Bioengineering and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland |
| Abstract: | |
| Keywords: | γ ‐secretase Alzheimer's disease transposon‐mediated multigene stable integration into genomic DNA membrane‐embedded multi‐protein complexes protein expression and purification |