Influence of poly(n‐isopropylacrylamide)–CNT–polyaniline three‐dimensional electrospun microfabric scaffolds on cell growth and viability |
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Authors: | Ashutosh Tiwari Yashpal Sharma Shinya Hattori Dohiko Terada Ashok K Sharma Anthony P F Turner Hisatoshi Kobayashi |
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Institution: | 1. Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Link?ping University, S‐58183 Link?ping, Sweden;2. International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1‐2‐1, Sengen, Tsukuba, Ibaraki 305‐0047, Japan;3. Department of Chemistry, G. J. University of Science and Technology, Hisar 125‐001, India;4. Department of Materials Science and Nanotechnology, D. C. R. University of Science and Technology, Murthal 131‐039, India;5. JST‐CREST, 5 Sanbancho, Chiyoda‐ku, Tokyo 102‐0075, Japan |
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Abstract: | This study investigates the effect on: (1) the bulk surface and (2) the three‐dimensional non‐woven microfabric scaffolds of poly(N‐isopropylacrylamide)–CNT–polyaniline on growth and viability of cells. The poly(N‐isopropylacrylamide)–CNT–polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non‐woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three‐dimensional scaffolds with OD: 400–500 μm; L: 6–20 cm. Mice fibroblast cells L929 were seeded on the both poly(N‐isopropylacrylamide)–CNT–polyaniline bulk surface as well as non‐woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N‐isopropylacrylamide)–CNT–polyaniline non‐woven microfabric matrices in compare to poly(N‐isopropylacrylamide)–CNT–polyaniline bulk and commercially available Matrigel? even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N‐isopropylacrylamide)–CNT–polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N‐isopropylacrylamide). The results suggest that poly(N‐isopropylacrylamide)–CNT–polyaniline non‐woven microfabrics could be used as a smart matrices for applications in tissue engineering. © 2012 Wiley Periodicals, Inc. Biopolymers 99: 334–341, 2013. |
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Keywords: | smart tissue engineering scaffolds conducting polymers carbon nanotubes responsive‐polymers biocompatible conducting matrices |
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