Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation |
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| Authors: | Atsushi Satomura Yoshiaki Katsuyama Natsuko Miura Kouichi Kuroda Ayako Tomio Takeshi Bamba Eiichiro Fukusaki Mitsuyoshi Ueda |
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| Affiliation: | 1. Div. of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, , Sakyo‐ku, Kyoto, 606‐8502 Japan;2. Dept. of Biotechnology, Graduate School of Engineering, Osaka University, , Suita, Osaka, 565‐0871 Japan |
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| Abstract: | A thermotolerant Saccharomyces cerevisiae yeast strain, YK60‐1, was bred from a parental strain, MT8‐1, via stepwise adaptation. YK60‐1 grew at 40°C, a temperature at which MT8‐1 could not grow at all. YK60‐1 exhibited faster growth than MT8‐1 at 30°C. To investigate the mechanisms how MT8‐1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress‐responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60‐1. Furthermore, nontargeting metabolome analysis showed that YK60‐1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8‐1. In conclusion, S. cerevisiae MT8‐1 acquired thermotolerance by induction of specific stress‐responsive genes and enhanced intracellular trehalose levels. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1116–1123, 2013 |
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| Keywords: | thermotolerant yeast breeding trehalose Saccharomyces cerevisiae |
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