Trehalose-producing operon treYZ from Arthrobacter ramosus S34 |
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Authors: | Yamamoto T Maruta K Watanabe H Yamashita H Kubota M Fukuda S Kurimoto M |
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Institution: | Hayashibara Biochemical Laboratories, Inc., Okayama, Japan. amaseken@hayashibara.co.jp |
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Abstract: | Arthrobacter ramosus S34, which produces trehalose from maltooligosaccharide, was isolated. A trehalose-producing operon, treYZ, was cloned from the genome. Expression experiments with treY and treZ confirmed that they coded malto-oligosyltrehalose synthase and malto-oligosyltrehalose trehalohydrolase, respectively. The amino acid sequence of TreY from A. ramosus S34 and that from Arthrobacter sp. Q36 did not show high identity, nor did those of TreZ. |
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