The Association of Plasminogen Activator Inhibitor Type 1 (PAI-1) Level and PAI-1 4G/5G Gene Polymorphism with the Formation and the Grade of Endometrial Cancer |
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Authors: | " target="_blank">Malik Ejder Yıldırım " target="_blank">Savas Karakuş " target="_blank">Hande Küçük Kurtulgan Hasan Kılıçgün " target="_blank">Serpil Erşan " target="_blank">Sevtap Bakır |
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Institution: | 1.Faculty of Medicine, Department of Medical Genetics,Cumhuriyet University,Sivas,Turkey;2.Faculty of Medicine, Department of Obstetrics and Gynecology,Cumhuriyet University,Sivas,Turkey;3.Faculty of Health Sciences, Department of Nutrition and Dietetic,Erzincan University,Erzincan,Turkey;4.Faculty of Medicine, Department of Medical Biochemistry,Cumhuriyet University,Sivas,Turkey |
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Abstract: | Plasminogen activator inhibitor type 1 (PAI-1) is a serine protease inhibitor (Serpine 1), and it inhibits both tissue plasminogen activator and urokinase plasminogen activator which are important in fibrinolysis. We aimed to find whether there is a possible association between PAI-1 level, PAI-1 4G/5G polymorphism, and endometrial cancer. PAI-1 levels in peripheral blood were determined in 82 patients with endometrial carcinoma and 76 female healthy controls using an enzyme-linked immunoassay (ELISA). Then, the genomic DNA was extracted and screened by reverse hybridization procedure (Strip assay) to detect PAI 1 4G/5G polymorphism. The levels of PAI-1 in the patients were higher statistically in comparison to controls (P < 0.001). The distribution of PAI-1 4G/5G polymorphism was quite different between patients and controls (P = 0.008), and 4G allelic frequency was significantly higher in the patients of endometrial cancer than in controls (P = 0.026). We found significant difference between Grade 1 and Grade 2+3 patients in terms of the PAI-1 levels (P = 0.047). There was no association between PAI-1 4G/5G polymorphism and the grades of endometrial cancer (P = 0.993). Our data suggest that the level of PAI-1 and PAI-1 4G/5G gene polymorphism are effective in the formation of endometrial cancer. PAI-1 levels are also associated with the grades of endometrial cancer. |
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