MicroRNA-215 enhances invasion and migration by targeting retinoblastoma tumor suppressor gene 1 in high-grade glioma |
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| Authors: | Yuzhen Wei Jianjing Sun Xingang Li |
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| Institution: | 1.Department of Neurosurgery,Qilu Hospital of Shandong University,Jinan,People’s Republic of China;2.Department of Neurosurgery,Jining No.1 People’s Hospital,Jining,People’s Republic of China;3.Department of Endocrinology,Jining No.1 People’s Hospital,Jining,People’s Republic of China;4.Brain Science Research Institute,Shandong University,Jinan,People’s Republic of China |
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| Abstract: | ObjectiveTo elucidate the molecular mechanism of microRNA-215 (miR-215) in the migration and invasion of high grade glioma.Results42 Patients were analysed for clinicopathological characteristics. qRT-PCR showed that miR-215 was up-regulated in glioma tissues compared with non-neoplastic brain tissues (P < 0.05). The up-regulated miR-215 was closely associated with high grade glioma (P < 0.01) and poor overall survival (P < 0.01). Transwell assay showed that re-expression of miR-215 enhanced migration and invasion of glioma cells. miR-215 also down-regulated retinoblastoma tumor suppressor gene 1 (RB1) expression by targeting its 3′-UTR. Reversely, re-expression of RB1 inhibited partial effect of miR-215 on migration and invasion in vitro.ConclusionsRe-expression of miR-215 promoted cell migration and invasion of glioma by targeting RB1. miR-215 can thus be used as a biomarker for tumor progression and prognosis in human high grade glioma. |
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