Iron Hematoxylin Chelates: II. Histochemistry of Myelin Sheath Stains |
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Authors: | Gene R. Berube Margaret M. Powers George Clark |
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Affiliation: | a Astxra Pharmaceutical Products, Inc., Neponset Street, Worcester, Massachusettsb Civil Aeromedical Research Institute, FAA, Oklahoma City, Okla |
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Abstract: | Aldehyde blockage, methylation, acetylation, amine blockage, and 4 min, 30 min and 24 hr deamination of paraffin sections of cat spinal cord were followed by staining with Lendrum's phloxine-tartrazine, Luxol fast blue MBS, PAS, phosphotungstic acid-hematoxylin, and Weil stains. Only the effects on staining of myelin are reported. These histochemical procedures separated the 5 stains into 3 groups: (1) phloxine-tartrazine and Luxol fast blue, (2) PAS, and (3) Weil and phosphotungstic acid-hematoxylin. The 3 patterns indicate that these stains may attach to 3 different reactive molecular sites in the sections. For the Weil and phosphotungstic acid-hematoxylin, such reactive sites are probably a secondary or tertiary amine or both. |
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