Toxicity monitoring of endocrine disrupting chemicals (EDCs) using freeze-dried recombinant bioluminescent bacteria |
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Authors: | Sung Woo Kim Sue Hyung Choi Jiho Min Man Bock Gu |
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Institution: | (1) Department of Environmental Science and Engineering and Advanced Environmental Monitoring Research Center (ADEMRC), Kwangju Institute of Science and Technology (K-JIST), 1 Oryong-dong, Puk-gu, 500-712 Kwangju, Korea |
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Abstract: | Five different freeze-dried recombinant bioluminescent bacteria were used for the detection of cellular stresses caused by
endocrine disrupting chemicals. These strains were DPD2794 (recA::luxCDABE), which is sensitive to DNA damage, DPD2540 (fabA::luxCDABE), sensitive to cellular membrane damage, DPD2511 (katG::luxCDABE), sensitive to oxidative damage, and TV1061 (grpE::luxCDABE), sensitive to protein damage. GC2, which emits bioluminescence constitutively, was also used in this study. The toxicity
of several chemicals was determined on the first four freeze-dried bacteria, while nonspecific cellular stresses were measured
using GC2. Damage caused by known endocrine disrupting chemicals, such as nonyl phenol, bisphenol A, and styrene, was detected
and classified according to toxicity mode, while others, such as phathalate and DDT, were not detected with the bacteria.
These results suggest that endocrine disrupting chemicals are toxic in bacteria, and do not act via an estrogenic effect,
and that toxicity monitoring and classification of some endocrine disrupting chemicals may be possible in the field using
these freeze-dried recombinant bioluminescent bacteria. |
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Keywords: | endocrine disrupting chemicals (EDCs) toxic effect freeze-dried recombinant bioluminescent bacteria cellular toxicity |
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