A comparative study of the membrane proteins from Naegleria species: A 23-kDa protein participates in the virulence of Naegleria fowleri |
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| Institution: | 1. Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. IPN 2508, Col. San Pedro Zacatenco, 07360, Mexico City, Mexico;2. Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. IPN 2508, Col. San Pedro Zacatenco, 07360, Mexico City, Mexico;3. Laboratorio de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, 07320, Mexico City, Mexico;1. School of Natural and Built Environment, Queen’s University Belfast, Belfast, BT7 1NN, United Kingdom;2. Laboratory of Soil Biodiversity, University of Neuchâtel, Neuchâtel, 2000, Switzerland;3. Real Jardin Botánico de Madrid, Spanish National Research Council, Madrid, E-28014, Spain;4. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria;5. Department of Zoology, Institute of Biosciences, University of São Paulo, São Paulo, 05508-090, Brazil;6. Botanical Garden of Neuchâtel, Neuchâtel, 2000, Switzerland;1. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, La Laguna, Tenerife, 38203 Islas Canarias, Spain;2. Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, 38203 Islas Canarias, Spain;3. Red de Investigación Colaborativa en Enfermedades Tropicales (RICET), Spain;1. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China;2. Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China;3. Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK;4. College of Life Sciences, Capital Normal University, Beijing, 100048, China;5. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, Shanghai, 201306, China;6. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Shanghai, 201306, China;7. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China;1. Institute of Evolution and Marine Biodiversity & Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China;2. Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou 510301, China;3. Department of Life Sciences, Natural History Museum, London SW7 5BD, United Kingdom;4. College of Life Sciences, Capital Normal University, Beijing 100048, China;1. Laboratory of Microbiota, College of Life Science, Northwest Normal University, Lanzhou 730070, China;2. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;3. College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, China;4. Department of Life Sciences, Natural History Museum, London SW7 5BD, UK;5. College of Life Sciences, Capital Normal University, Beijing 100048, China |
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| Abstract: | The plasma membrane is essential in the pathogenicity of several microorganisms. However, to date, there are few studies related to the plasma membrane proteins in Naegleria fowleri; this amoeba produces a fatal disease called primary amoebic meningoencephalitis. In the present study, we analyzed the electrophoretic pattern of the membrane proteins of N. fowleri and compared it with the nonpathogenic N. lovaniensis and N. gruberi. We detected a 23-kDa protein (Nf23) present at a higher level in N. fowleri than in the nonpathogenic amoebae. The mass spectrometry analysis showed that the Nf23 protein has a sequence of 229 amino acids that corresponds to a membrane protein. The mRNA level of nf23 was overexpressed 4-fold and 40,000-fold in N. fowleri compared with N. lovaniensis and N. gruberi, respectively. Moreover, we found a 5-fold overexpression of nf23 in N. fowleri trophozoites recovered from mouse brains compared with trophozoites axenically cultivated. In addition, the cytopathic effect on Madin-Darby Canine Kidney cells coincubated with N. fowleri diminished in the presence of antibodies against Nf23; nevertheless, the nonpathogenic amoebae did not produce damage to the monolayer cells. These results suggest that the plasma membrane protein Nf23 is probably involved in the virulence of N. fowleri. |
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| Keywords: | Comparative expression Membrane protein |
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