Cytotoxic and Antimigratory Activity of Retrochalcones from Glycyrrhiza echinata L. on Human Cancer Cells |
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Authors: | Dicle Çevik Suat Erdogan Riza Serttas Yüksel Kan Hasan K?rm?z?bekmez |
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Institution: | 1. Department of Pharmacognosy, Faculty of Pharmacy, Trakya University, 22030, Balkan Campus Edirne, Turkey;2. Department of Medical Biology, School of Medicine, Trakya University, 22030, Balkan Campus Edirne, Turkey;3. Department of Medicinal Plants, Faculty of Agriculture, Selçuk University, 42070 Konya, Turkey;4. Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, 34755 Kay??da??, ?stanbul, Turkey |
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Abstract: | Cytotoxic activity-guided fractionation studies on Glycyrrhiza echinata roots led to the isolation of eight compounds ( 1 – 8 ). Chemical structures of the isolates were identified by NMR and MS analysis. Among the tested molecules, retrochalcones namely echinatin ( 3 ) (IC50=23.45–41.83 μM), licochalcone B ( 4 ) (IC50=36.04–39.53 μM) and tetrahydroxylmethoxychalcone ( 5 ) (IC50=7.09–80.81 μM) were the most active ones against PC3, MCF7 and HepG2 cells. Moreover, 5 exhibited selectivity on prostate cancer cells (SI: 5.19). Hoechst staining and Annexin V/PI binding assays as well as cell cycle analysis on the compounds 3 (23 μM) and 5 (5 and 7 μM) demonstrated that these retrochalcones induced apoptosis and significantly suppressed cell cycle in G1 and G2/M phases. Furthermore, 3 and 5 showed antimigratory effects on PC3 cells by wound healing assay. The results indicated that tested retrochalcones most particularly 5 could be potential anticancer drug candidates that prevent proliferation and migration of cancer cells. |
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Keywords: | Glycyrrhiza echinata bioassay-guided isolation retrochalcone cytotoxic activity apoptosis |
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