Cyclophosphamide induced oxidative stress,lipid per oxidation,apoptosis and histopathological changes in rats: Protective role of boron |
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| Affiliation: | 1. Siirt University, Department of Elementary Education, Faculty of Education, Siirt, Turkey;2. Eskişehir Osmangazi University, Faculty of Medicine, Department of Histology and Embryology, Eskişehir, Turkey;3. Mardin Artuklu University, Vocational Higher School of Health Services, Department of Medical Services and Techniques, Mardin, Turkey;4. Kırıkkale University, Vocational School of Health Services, Kırıkkale, Turkey;5. Karabük University, Faculty of Medicine Department of Medical Pharmacology, Karabük, Turkey;6. Karabük University, Faculty of Health Sciences, Karabuk, Turkey;7. Ryerson University, Faculty of Science, Toronto, Ontario, Canada;8. Eskisehir Osmangazi University, Faculty of Science and Letters, Department of Biology, TR-26480 Eskisehir, Turkey;1. Karadeniz Technical University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Trabzon, Turkey;2. Karadeniz Technical University, Drug and Pharmaceutical Technology Application and Research Center, Trabzon, Turkey;1. Department of Biomedical Sciences, Pharmacology Division, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia;2. Department of Internal Medicine, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia;3. Department of Biomedical Sciences, Histopathology Division, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia;1. Institute for Genetic Engineering and Biotechnology, Laboratory for Cytogenetics and Genotoxicology, University of Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and Herzegovina;2. Veterinary Faculty, University of Sarajevo, Department of Pathology and Department of Pharmacology and Toxicology, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina;3. Faculty of Science, Department of Chemistry, University of Sarajevo, Zmaja od Bosne 35, 71 000 Sarajevo, Bosnia and Herzegovina;1. Biochemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Eini Street, Cairo 11562, Egypt;2. Phytochemistry Department, National Research Center, P.C. (12622), Dokki, Giza, Egypt |
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| Abstract: | BackgroundCyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation.MethodsThis current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis.ResultsThe damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased.ConclusionB significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties. |
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| Keywords: | Cyclophosphamide Testicular damage Apoptosis Boron Anti-Oxidant Lipid per oxidation |
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