Screening of hub genes and prediction of putative drugs in arsenic-related bladder carcinoma: An in silico study |
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| Affiliation: | 1. Clinical Medical College, Dali University, Dali, Yunnan, China;2. Affiliated Hospital of Guizhou Medical University, Guiyang, China;3. Department of Oncology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China;4. Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China;1. College of Science, Al-Qasim Green University, Iraq;2. Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt;3. National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, China;1. Synovial Fluid Laboratory, National Institute of Rehabilitation “Luis Guillermo Ibarra Ibarra”, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;2. Orthopedic Sports Medicine and Arthroscopy Service, National Institute of Rehabilitation, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;3. Department of Natural Resources, Geophysics Institute-UNAM. Circuito de la investigación Científica s/n, Ciudad Universitaria, Cd. Universitaria, C.P. 04150, Mexico City, Mexico;4. ICP-MS Laboratory, Geophysics Institute-UNAM. Circuito de la investigación Científica s/n, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico;5. Microscopy Laboratory, National Institute of Rehabilitation “Luis Guillermo Ibarra Ibarra”, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;6. Tissue Engineering and Cell Therapy and Regenerative Medicine Unit, National Institute Rehabilitation “Luis Guillermo Ibarra Ibarra”, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;7. Department of Adult Joint Reconstruction, National Rehabilitation Institute, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;8. Renal Toxicology Laboratory, Cinvestav, Av. Politécnico Nacional 2508, San Pedro Zacatenco, C.P. 07360, Mexico City, Mexico;9. Department of Toxicology, Cinvestav. Av. Politécnico Nacional 2508, San Pedro Zacatenco, C.P. 07360, Mexico City, Mexico;10. Department of Animal Nutrition, National Institute of Medical Sciences and Nutrition Salvador Zubiran. Vasco de Quiroga 15, Belisario Domínguez Secc. 16, C.P. 14080, Mexico City, Mexico;1. Department of Fisheries and Diseases, Faculty of Veterinary Medicine, Aksaray University, Turkey;2. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Aksaray University, Turkey;3. Department of Veterinary Histology and Embryology, Faculty of Veterinary Medicine, Aksaray University, Turkey;1. Key Laboratory of Trace Element Nutrition of National Health Commission of China, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, 27 Nanwei Road, Beijing 100050, China;2. Animal Laboratory, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 29 Nanwei Road, Beijing 100050, China;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China;2. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China;3. International Joint Research Laboratory for Probiotics at Jiangnan University, Wuxi, Jiangsu, 214122, China;4. National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu, 214122, China;5. (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, China;6. Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology & Business University, Beijing, 100048, China |
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| Abstract: | BackgroundEvidence showed that inorganic arsenic (iAs) can trigger malignant transformation in cells with complex mechanisms. Thus, we aimed to investigate the possible molecules, pathways and therapeutic drugs for iAs-induced bladder cancer (BC) by using bioinformatics approaches.MethodsMicroarray-based data were analyzed to screen the differentially expressed genes (DEGs) between iAs-related BC cells and controls. Then, the roles of DEGs were annotated and the hub genes were screened out by protein-protein interaction network. The key genes were further selected from the hub genes through an assessment of the prognostic values. Afterward, potential drugs were predicted by using CMAP analysis.ResultsAnalysis of a dataset (GSE90023) generated 21 upregulated and 47 downregulated DEGs, which were enriched in various signaling pathways. Among the DEGs, four hub genes including WNT7B, SFRP1, DNAJB2, and ATF3, were identified as the key genes because they might predict poor prognosis in BC patients. Lastly, Cantharidin was predicted to be a potential drug reversing iAs-induced malignant transformation in urinary epithelium cells.ConclusionThe present study found several hub genes involved in iAs-induced malignant transformation in urinary epithelium cells, and predicted several small agents for iAs toxicity prevention or therapy. |
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| Keywords: | Inorganic arsenic Differentially expressed genes Carcinogenesis Bladder carcinoma Bioinformatics |
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