Bromelain from Ananas comosus stem attenuates oxidative toxicity and testicular dysfunction caused by aluminum in rats |
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| Institution: | 1. College of Science, Al-Qasim Green University, Iraq;2. Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt;3. National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, China;1. CONACYT— National Institute of Cardiology “Ignacio Chávez”, Juan Badiano 1, Sección XVI, Tlalpan, CDMX, 14080, Mexico;2. Neurochemistry Department, National Institute of Neurology and Neurosurgery “Manuel Velasco Suárez”, Insurgentes sur 3877, La Fama, Tlalpan, CDMX, 14269, Mexico;3. Pharmacology Department, National Institute of Pediatrics, Iman Avenue 1, Insurgentes Cuicuilco, Coyoacán, CDMX, 04530, Mexico;1. Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran;2. Department of Biochemistry and Genetics, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran;3. Department of Laboratory Sciences, Faculty of Paramedicine, Arak University of Medical Sciences, Arak, Iran;4. Department of Biochemistry, Khomein University of Medical Sciences, Khomein, Iran;5. Department of Biostatistics and Epidemiology, School of Health, Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran;6. Community and Preventive Medicine Specialist, Department of community medicine, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran;7. Department of Pharmacology, School of Medicine, Arak University of Medical Sciences, Arak, Iran;1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China;2. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China;3. Henan Key Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, Henan, 467036, China;4. School of Pharmaceutical Science and Technology, Suzhou Chien-shiung Institute of Technology, Taicang, 215400, China;1. Universidad de Antioquia, Grupo de Biotransformación – Escuela de Microbiología, Calle 70 No 52-21, A.A 1226 Medellín, Colombia;2. Instituto de Ciencia y Tecnología Alimentaria, INTAL, Carrera. 50 G # 12 Sur 91, Itagüí, Colombia;3. Universidad CES, Facultad de Ciencias y Biotecnología, Calle 10 A No. 22 – 04, Medellín, Colombia |
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| Abstract: | BACKGROUNDAluminum (Al) has been reported to induce testicular injury via oxidative stress. Ananas comosus stem extract is an inexpensive byproduct waste rich in bromelain which is a group of sulfur-containing enzymes known for its biological activities and medicinal applications. So, the current investigation aims to evaluate the efficacy of bromelain in counteracting oxidative injury and testicular dysfunction stimulated by aluminum in rats.METHODSMale adult Wistar rats were divided into four groups. The first group used as control, however, the second and third groups were received bromelain (250 mg/kg) and AlCl3 (34 mg/Kg, 1/25 LD50), and the fourth group supplemented with bromelain one hour before AlCl3 intoxication, respectively. Bromelain was administered daily while AlCl3 was given every other day by oral gavages for one month.RESULTSAl intoxicated animals revealed an elevation in lipid peroxidation (TBARS and H2O2) level and lactate dehydrogenase (LDH) activity. However, reduced glutathione (GSH) and protein contents, antioxidant enzymes (SOD, CAT, GPx, GR, GST), phosphatases (ALP, AcP) and aminotransferases (AST, ALT) activities were significantly reduced. Additionally, considerable amendments in hormonal levels (testosterone, luteinizing and follicle-stimulating hormone) and sperm characteristics were spotted. Further, histological variations in the testes section were detected and this supports the biochemical observations. Otherwise, rats supplemented with bromelain alone diminished TBARS and H2O2 and augmented mostly other parameters. Furthermore, supplementation with bromelain before Al intoxication in rats exhibited worthy betterment in oxidative stress markers, hormones, and sperm quality compared to Al treated group.CONCLUSIONIn conclusion, bromelain had a powerful protective role against Al-induced testicular dysfunction so, it represents a novel approach in metal toxicity processing. |
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| Keywords: | Aluminum chloride Bromelain Oxidative stress Antioxidant enzymes Testicular dysfunction |
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