Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro
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| Authors: | Bruno Corrêa Bellagamba Bianca Regina Ribas de Abreu Ivana Grivicich Carolina Franke Markarian Eduardo Chem Melissa Camassola Nance Beyer Nardi Rafael Rodrigues Dihl |
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| Affiliation: | 1.Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;2.Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada à Saúde, Universidade Luterana do Brasil, Canoas, RS, Brazil;3.Complexo Hospitalar Santa Casa de Porto Alegre, Porto Alegre, RS, Brazil |
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| Abstract: | Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS, which was not observed when OVCAR-3 cells were exposed to this drug. |
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| Keywords: | Mesenchymal stem cells Cisplatin MTT Comet assay OVCAR-3 cells |
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