In-vitro evaluation of copper/copper oxide nanoparticles cytotoxicity and genotoxicity in normal and cancer lung cell lines |
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| Institution: | 1. Biophysics Department, Faculty of Science, Cairo University, Egypt;2. Faculty of Physical Therapy, Modern University for Science and Technology (MTI), Egypt;3. Faculty of Engineering, British University in Egypt (BUE), Egypt;1. Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 83 Huddinge, Stockholm, Sweden;2. KTH Royal Institute of Technology, Division of Surface and Corrosion Science, School of Chemical Science and Engineering, SE-100 44 Stockholm, Sweden;3. Department of Laboratory Medicine, Karolinska Institutet, SE-141 83 Huddinge, Stockholm, Sweden;1. Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Egypt;2. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Alexandria University, Egypt;3. Department of Pathology, Faculty of Veterinary Medicine, Alexandria University, Egypt;1. Finnish Institute of Occupational Health, Helsinki, Finland;2. Department of Anatomy Embryology and Genetics, University of Zaragoza, 50009 Zaragoza, Spain;1. Nanomedicine & Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia;2. King Abdullah Institute for Nanotechnology, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia;3. Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, PO Box 2455, Riyadh, Saudi Arabia;4. Colorectal Research Centre, College of Medicine, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia |
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| Abstract: | BackgroundNanotoxicology is a major field of study that reveals hazard effects of nanomaterials on the living cells.MethodsIn the present study, Copper/Copper oxide nanoparticles (Cu/CuO NPs) were prepared by the chemical reduction method and characterized by different techniques such as: X-Ray Diffraction, Transmission and Scanning Electron Microscopy. Evaluation of the toxicity of Cu/CuO NPs was performed on 2 types of cells: human lung normal cell lines (WI-38) and human lung carcinoma cell (A549). To assess the toxicity of the prepared Cu/CuOs NPs, the two cell types were exposed to Cu/CuO NPs for 72 h. The half-maximal inhibitory concentration IC50 of Cu/CuO NPs for both cell types was separately determined and used to examine the cell genotoxicity concurrently with the determination of some oxidative stress parameters: nitric oxide, glutathione reduced, hydrogen peroxide, malondialdehyde and superoxide dismutase.ResultsCu/CuO NPs suppressed proliferation and viability of normal and carcinoma lung cells. Treatment of both cell types with their IC50’s of Cu/CuO NPs resulted in DNA damage besides the generation of reactive oxygen species and consequently the generation of a state of oxidative stress.ConclusionOverall, it can be concluded that the IC50's of the prepared Cu/CuO NPs were cytotoxic and genotoxic to both normal and cancerous lung cells. |
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| Keywords: | Copper/Copper oxide nanoparticles (Cu/CuONPs) Human lung normal cell lines (WI-38 cells) Human lung carcinoma cell lines (A549 cells) Toxicity Oxidative stress Comet assay DNA |
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