Enzymatic Substrate Inhibition in Metal Free Catecholase Activity |
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Authors: | Kuntal Pal Souvik Barman Jayanta Bag |
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Institution: | Department of Chemistry, University of Calcutta, 92 A.P.C Road, Kolkata, 700009 India |
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Abstract: | The catecholase activities were routinely modeled using transition metal complexes as catalyst and in some case basic pH were used as a reaction condition. In this article, the catalytic aerobic oxidation of proxy substrate 3,5-di-tert-butylcatechol (DTBC) in methanol using triethylamine/diethylamine as catalyst was demonstrated as a functional mimic of catecholase activity. The kinetic manifestation of DTBC oxidation was explained as enzymatic substrate inhibition pattern in Michaelis-Menten kinetic model. The mechanistic insight of the aerobic oxidation of DTBC was further validated using various spectroscopic techniques and DFT methods. |
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Keywords: | catecholase activity metal free substrate inhibition 3 5-di-tert-butylcatechol cyclic voltammetry DFT methods |
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