A single-cell view of spermatogonial stem cells |
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| Affiliation: | 1. Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Diego, La Jolla, CA, 92093, USA;2. Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA, 92093, USA;1. Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92093, USA;2. Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA;1. Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan;2. Japan Science and Technology Agency, PRESTO, Kyoto 606-8501, Japan;3. The Institute for Physical and Chemical Research (RIKEN), Bioresource Center, Tsukuba 305-0074, Japan;4. Department of Pathology, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan;1. School of Molecular Biosciences, Centre for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA;1. Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, PRC;2. Department of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC;3. Guangdong Key Laboratory of Genome Instability and Human Disease Prevention, Department of Biochemistry and Molecular Biology, Shenzhen University Health Science Center, Shenzhen, Guangdong 518060, PRC;4. Reproductive Medicine Center of The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, PRC;5. Biomedical Pioneering Innovation Center and Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC;6. Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Peking University, Beijing 100191, PRC;7. Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, PRC;8. Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangdong 510515, PRC;1. Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Baltimore, MD 21205, USA;2. Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA;3. School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman WA 99164, USA;1. Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA;2. Genomics Core, University of Texas at San Antonio, San Antonio, TX 78249, USA;3. Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USA;4. Department of Anatomy & Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA;5. East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA;6. The UT Transplant Center, UT Health San Antonio, San Antonio, TX 78229, USA;7. The Infertility Center of St. Louis, Chesterfield, MO 63017, USA |
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| Abstract: | Spermatogonial stem cells (SSCs) are essential for long-term spermatogenesis and are the subject of considerable clinical interest, as ‘SSC therapy’ has the potential to cure some forms of male infertility. Recently, we have learned more about SSCs and spermatogenesis in general from a plethora of studies that performed single-cell RNA sequencing (scRNAseq) analysis on dissociated cells from human, macaque, and/or mice testes. Here, we discuss what scRNAseq analysis has revealed about SSC precursor cells, the initial generation of SSCs during perinatal development, and their heterogeneity once established. scRNAseq studies have also uncovered unexpected heterogeneity of the larger class of cells that includes SSCs — undifferentiated spermatogonia. This raises the controversial possibility that multiple SSC subsets exist, which has implications for mechanisms underlying spermatogenesis and future SSC therapeutic approaches. |
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| Keywords: | Spermatogonial stem cells Spermatogonia Prospermatogonia Spermatogenesis Testis Heterogeneity Single-cell RNAseq |
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