Protections against toxicity in the brains of rat with chronic fluorosis and primary neurons exposed to fluoride by resveratrol involves nicotinic acetylcholine receptors |
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| Affiliation: | 1. Departments of Pathology at Guizhou Medical University and the Affiliated Hospital of Guizhou Medical University, PR China;2. Key Laboratory of Endemic and Ethnic Diseases, (Guizhou Medical University) of the Ministry of Education, PR China;3. Provincial Key Laboratory of Medical Molecular Biology, Guiyang, 550004, Guizhou, PR China;1. Univ Lyon, Univ Jean Monnet, INSERM, U 1059 SainBioSE, F-42023 Saint-Etienne, France;2. University Hospital Saint-Etienne, Department of Gynecology and Obstetrics, F-42055 Saint-Etienne, France;3. Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, F-42023 Saint-Etienne, France;1. Environmental Toxicology Unit, Department of Biochemistry, Faculty of Science, University of Port Harcourt, P.M.B 5323, Choba, Rivers State, Nigeria;2. Department of Physiology, College of Medicine, Rivers State University, Port Harcourt, P.M.B 5080, Rivers State, Nigeria;1. Key Laboratory of Endemic and Ethnic Diseases (Guizhou Medical University), Ministry of Education, PR China;2. Key Laboratory of Medical Molecular Biology (Guizhou Medical University), Guiyang 550004, PR China;3. Department of Pathology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, PR China;1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University. Taigu, Shanxi 030801, PR China;2. Shanxi Key Laboratory of Environmental Veterinary Medicine, Shanxi Agricultural University. Taigu, Shanxi 030801, PR China;3. College of Arts and Sciences, Shanxi Agricultural University. Taigu, Shanxi 030801, PR China;4. College of Life Sciences, Shanxi Agricultural University. Taigu, Shanxi 030801, PR China |
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| Abstract: | Protection of Resveratrol (RSV) against the neurotoxicity induced by high level of fluoride was investigated. Sprague-Dawley (SD) rats and their offspring, as well as cultures of primary neurons were divided randomly into four groups: untreated (control); treated with 50 mg RSV/kg/ (once daily by gavage) or (20 M in the cultured medium); exposed to 50 ppm F− in drinking water or 4 mmol/l in the cultured medium; and exposed to fluoride then RSV as above. The adult rats were treated for 7 months and the offspring sacrificed at 28 days of age; the cultured neurons for 48 h. For general characterization, dental fluorosis was assessed and the fluoride content of the urine measured (by fluoride-electrode) in the rates and the survival of cultured neurons monitored with the CCK-8 test. The spatial learning and memory of rats were assessed with the Morris water maze test. The levels of α7 and α4 nicotinic acetylcholine receptors (nAChRs) were quantified by Western blotting; and the activities of superoxide dismutase (SOD) and catalase (CAT), and the levels of malondialdehyde (MDA) and H2O2 assayed biochemically. The results showed that chronic fluorosis resulted in the impaired learning and memory in rats and their offspring, and more oxidative stress in both rat brains and cultured neurons, which may be associated the lower levels of α7 and α4 nAChR subunits. Interestingly, RSV attenuated all of these toxic effects by fluorosis, indicating that protection against the neurotoxicity of fluoride by RSV might be in mechanism involved enhancing the expressions of these nAChRs. |
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| Keywords: | Fluorosis Learning and memory Nicotinic acetylcholine receptors Oxidative stress Resveratrol |
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