GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod |
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Authors: | Jaysing Mahavirsing Dinore Harshal Shivaji Patil Samreen Farooqui Vidya Pradhan Mazahar Farooqui |
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Institution: | 1. Assistant Professor, Department of Chemistry, Indraraj Arts, Commerce and Science College, Sillod, Aurangabad, Maharashtra, 431112 India;2. Assistant Professor, Department of Chemistry, Moreshwar Arts, Commerce and Science College, Bhokardan, Jalna, Maharashtra, 431114 India;3. Assistant Professor, Post Graduate and Research Center, Maulana Azad College, Aurangabad, Maharashtra, 431001 India;4. Associate Professor. Dr. Rafiq Zakaria College for Women, Aurangabad, Maharashtra, 431001 India;5. Professor, Post Graduate and Research Center, Maulana Azad College, Aurangabad, Maharashtra, 431001 India |
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Abstract: | Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty-four compounds. GC/MS chromatogram analysis suggested the presence of opioid α-N-Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti-fungal, and anti-oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 μg/mL similar to the standard Ampicillin (MIC 100 μg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 μg/mL. The pod extract showed significant DPPH scavenging activity (IC50=78.38±0.15) which suggests its antioxidant potential. |
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Keywords: | Vigna unguiculata cowpea GC/MS LC/MS antimicrobial activity antioxidant activity |
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