Accessing the transcriptional status of selenoproteins in skin cancer-derived cell lines |
| |
| Affiliation: | 1. Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil;2. Laboratory of Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil;1. Sarayönü Vocational High School, Selçuk University, 42430 Konya, Turkey;2. Advanced Technology Research and Application Center, Selçuk University, 42075 Konya, Turkey;3. Department of Physics, Faculty of Science, Selçuk University, 42075 Konya, Turkey;1. King’s College London, Department of Nutritional Sciences, School of Life Course Sciences, Metal Metabolism Group, London, UK;2. Kings College London, Centre for Developmental Neurobiology, London, UK;1. Department of Medical Genetics, Changzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, No. 16 Ding Xiang Road, Changzhou, Jiangsu Province, China;2. Department of Laboratory Medicine, Changzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, No. 16 Ding Xiang Road, Changzhou, Jiangsu Province, China;3. Department of Obstetrics and Gynecology, Changzhou Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, No. 16 Ding Xiang Road, Changzhou, Jiangsu Province, China;1. Univ Lyon, Univ Jean Monnet, INSERM, U 1059 SainBioSE, F-42023 Saint-Etienne, France;2. University Hospital Saint-Etienne, Department of Gynecology and Obstetrics, F-42055 Saint-Etienne, France;3. Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, F-42023 Saint-Etienne, France |
| |
| Abstract: | BackgroundSelenoproteins are selenocysteine (Sec)-containing proteins that exhibit numerous physiological functions, mainly antioxidative activities. Studies have suggested that several human selenoproteins play an important role in tumor initiation and progression, including melanoma.MethodsUsing RNA-seq data set from Sequence Reads Archive (SRA) experiments published at the National Center for Biotechnology Information (NCBI), we determined and compared the transcriptional levels of the 25 selenoproteins-coding sequences found in 16 human-derived melanoma cell lines and compared to four melanocyte controls.Results15 selenoprotein-coding genes were found to be expressed in melanoma and normal melanocyte cells, and their mRNA levels varied among the cell lines. All melanoma cells analyzed with BRAF or NRAS mutations presented upregulated levels of SELENOI, TXNRD1, and SELENOT transcripts and downregulated levels of SELENOW and SELENON transcripts in comparison with melanocytes controls. Moreover, SELENOW, SELENON, SELENOI, TXNRD1, and SELENOT-coding transcripts were affected when BRAF-mutated A375 cells were treated with CPI203, A771726 or Vorinostat drugs.ConclusionOur results indicate that melanoma cells can modify, in a different manner, the selenoprotein transcript levels, as a possible mechanism to control tumor progression. We suggest that the usage of diet and supplements containing selenium should be carefully used for patients with melanoma. |
| |
| Keywords: | Selenoproteins Melanoma RNA-seq |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
