Biochemical and histomorphological findings in Swiss Wistar rats treated with potential boron-containing therapeutic - K2[B3O3F4OH] |
| |
| Institution: | 1. Institute for Genetic Engineering and Biotechnology, Laboratory for Cytogenetics and Genotoxicology, University of Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and Herzegovina;2. Veterinary Faculty, University of Sarajevo, Department of Pathology and Department of Pharmacology and Toxicology, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina;3. Faculty of Science, Department of Chemistry, University of Sarajevo, Zmaja od Bosne 35, 71 000 Sarajevo, Bosnia and Herzegovina;1. Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, 96813, USA;2. Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo – FMRP/USP, Brazil;1. School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, 550025, China;2. Guizhou Orthopedics Hospital, Guiyang, 550007, China;1. Clinical Medical College, Dali University, Dali, Yunnan, China;2. Affiliated Hospital of Guizhou Medical University, Guiyang, China;3. Department of Oncology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China;4. Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China;1. Key Laboratory of Trace Element Nutrition of National Health Commission of China, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, 27 Nanwei Road, Beijing 100050, China;2. Animal Laboratory, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 29 Nanwei Road, Beijing 100050, China;1. Synovial Fluid Laboratory, National Institute of Rehabilitation “Luis Guillermo Ibarra Ibarra”, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;2. Orthopedic Sports Medicine and Arthroscopy Service, National Institute of Rehabilitation, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;3. Department of Natural Resources, Geophysics Institute-UNAM. Circuito de la investigación Científica s/n, Ciudad Universitaria, Cd. Universitaria, C.P. 04150, Mexico City, Mexico;4. ICP-MS Laboratory, Geophysics Institute-UNAM. Circuito de la investigación Científica s/n, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico;5. Microscopy Laboratory, National Institute of Rehabilitation “Luis Guillermo Ibarra Ibarra”, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;6. Tissue Engineering and Cell Therapy and Regenerative Medicine Unit, National Institute Rehabilitation “Luis Guillermo Ibarra Ibarra”, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;7. Department of Adult Joint Reconstruction, National Rehabilitation Institute, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico;8. Renal Toxicology Laboratory, Cinvestav, Av. Politécnico Nacional 2508, San Pedro Zacatenco, C.P. 07360, Mexico City, Mexico;9. Department of Toxicology, Cinvestav. Av. Politécnico Nacional 2508, San Pedro Zacatenco, C.P. 07360, Mexico City, Mexico;10. Department of Animal Nutrition, National Institute of Medical Sciences and Nutrition Salvador Zubiran. Vasco de Quiroga 15, Belisario Domínguez Secc. 16, C.P. 14080, Mexico City, Mexico;1. Institute of Immunology, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, D-52074 Aachen, Germany;2. Institute for Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, D-52074 Aachen, Germany |
| |
| Abstract: | BackgroundBoron and boron containing compounds are known for their biological and protective roles being non-toxic and non-mutagenic in low concentrations. Male rats were exposed to halogenated boroxine (HB), dipotassium-trioxohydroxytetrafluorotriborate K2B3O3F4OH], a potential new boron-containing therapeutic, aiming to determine concentrations with no adverse effects on selected serum biochemical parameters and histomorphological features.MethodsHB was prepared by reacting potassium hydrofluoride (KHF2) with boric acid in molar ratios 2:3 at room temperature and its primary structure contains 4 fluorine atoms substituted in 6-membered ring. In concentrations of 10, 25, 35 and 45 mg/kg, HB was administered intraperitoneally as a single dose. Biochemical parameters were observed 24 and 96 h following the treatment. Effects of HB on biochemical blood parameters were also observed 24 h following continuous nine days application in concentrations of 10 mg/kg intraperitoneally and 50 mg/kg per os. Histomorphological observation of kidneys, liver, spleen, lungs and heart was performed for all treated animals.ResultsAdministration of single high dose of HB (35 mg/kg–45 mg/kg) effected high levels of urea and creatinine, which indicated renal injury that appeared to be temporary. Possible cause of concern is pancreatic injury indicated by elevated levels of serum amylase in the groups of animals that received the highest dosages of the substance. Histopathological examination of selected tissues revealed mild to moderate lesions in the kidneys and livers associated with administration of HB.ConclusionObservation of biochemical serum parameters or histopathology of examined tissues revealed no adverse effects of HB either after the administration of single dose lower than 35 mg/kg or following repeated administration at 10 mg/kg. These dosages should be further considered for potential therapeutic applications. |
| |
| Keywords: | Boron containing compound Halogenated boroxine Blood parameters Kidney Liver |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
