Determination of the Effects of Bee Venom on Triple Negative Breast Cancer Cells in Vitro |
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Authors: | Sedat Sevin Asuman Deveci Ozkan Hidayet Tutun Ibrahim Kivrak Ozge Turna Gamze Guney Eskiler |
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Institution: | 1. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, 06070 Ankara, Turkey;2. Department of Medical Biology, Faculty of Medicine, Sakarya University, 54050 Sakarya, Turkey;3. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, 15030 Burdur, Turkey;4. Muğla Sıtkı Koçman University -, Muğla Vocational School Department of Chemistry and Chemical Treatment Technologies, 48000 Muğla, Turkey;5. Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Istanbul Cerrahpasa University, 34320 Istanbul, Turkey |
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Abstract: | Honeybees provide multiple products such as bee venom (BV) which are used for various nutritional and medicinal purposes. BV has received great attention due to its wide range of bioactive components with potential anti-cancer effects on different cancers. Triple negative breast cancer (TNBC) is defined as an aggressive type of breast cancer and new therapeutic targets are required for its treatment. In the current literature information is varied about the composition and quantity of BV bioactive compounds as well as the origin of BV and its significance. In this context, the cytotoxic and apoptotic effects of BV with a higher rate of mellitin from Apis mellifera anatoliaca (Muğla ecotype) on MDA-MB-231 cells was evaluated, in vitro. The cytotoxic, apoptotic and morphological effects of BV were determined by WST-1, Annexin V, cell cycle analysis and Acridine Orange staining. The results showed that BV caused apoptotic cell death in TNBC cells at a lower dose (0.47 μg/mL, p<0.01). This study suggests that BV could be developed as a potential therapeutic agent for cancer treatment. However, the mechanism of BV-induced apoptosis death should be clarified at the molecular level. |
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Keywords: | apoptosis bee venom honey bee triple negative breast cancer (TNBC) |
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