Embryogenic callus formation from maize protoplasts |
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Authors: | K K Kamo K L Chang M E Lynn T K Hodges |
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Institution: | (1) Department of Botany and Plant Pathology, Purdue University, 47907 West Lafayette, IN, USA |
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Abstract: | Maize (Zea mays L.) protoplasts have been obtained which divide rapidly and produce a callus that differentiates to form somatic embryos. The somatic embryos can be induced to form roots and small leaf-like structures. The genotype was the hybrid A188xBlack Mexican Sweet. Protoplasts were prepared from an embryogenic suspension culture derived from a Type II callus which had been selected from Type I callus produced by immature zygotic embryos. The basal medium for the suspension culture was N6 (C.C. Chu et al., 1975, Scientia Sinica 18, 659–668). The 2,4-dichlorophenoxyacetic acid concentration of the suspension culture was critical for subsequent protoplast growth and was optimal at 4.0 mg.l. Protoplasts had to be cultured in a low-osmoticum medium (0.3 M mannitol) for subsequent cell divisions to occur. The protoplasts have been transformed transiently with the gene chloramphenicol acetyltransferase (CAT) containing the 35S promoter obtained from cauliflower mosaic virus (CaMV-35S).Abbreviations FDA
fluorescein diacetate
- ABA
abscisic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid |
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Keywords: | Embryonic callus Protoplast Zea (embryogenic callus) Transformation (protoplasts) |
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