| 假单胞菌属No.2120生产D-甘露糖异构酶发酵培养基的优化 |
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| 引用本文： | 张荆,童群义. 假单胞菌属No.2120生产D-甘露糖异构酶发酵培养基的优化[J]. 生物加工过程, 2010, 8(1): 34-39 |
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| 作者姓名： | 张荆 童群义 |
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| 作者单位： | 江南大学,食品学院,食品科学与技术国家重点实验室,无锡,214122 |
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| 摘 要： | 通过单因子实验、Plackett-Burman实验设计、响应面分析法对假单胞菌属No.2120产D-甘露糖异构酶的培养基进行优化,确定发酵优化条件:果糖15.26 g/L,牛肉膏20 g/L,酵母膏2 g/L,K2HPO42 g/L,MgSO4.7H2O0.5 g/L,NaCl 0.5 g/L,Tween-80 1.54 g/L。采用优化配方异构酶比酶活可以达到68.28 U/mL。
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| 关 键 词： | 异构酶 D-甘露糖 优化 假单胞菌属 |
Optimization of fermention culture medium for producing D-mannose isomerose by Pesudomonas sp.No.2120 |
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| ZHANG Jing,TONG Qun-yi. Optimization of fermention culture medium for producing D-mannose isomerose by Pesudomonas sp.No.2120[J]. Chinese Journal of Bioprocess Engineering, 2010, 8(1): 34-39 |
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| Authors: | ZHANG Jing TONG Qun-yi |
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| Affiliation: | ZHANG Jing,TONG Qun-yi(State Key Laboratory of Food Science , Technology,School of Food Science , Technology,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | In order to increase the production of D-mannose isomerase,the culture medium of Pesudomonas sp.No.2120 was optimized through experiments by single-factor design,Plackett-Burman design,and response surface methodology.The optimum medium was as follows(g/L): fructose 15.26,beef extract 20,yeast extract 2,K2HPO4 2,MgSO4 7H2O 0.5,NaCl 0.5,and Tween-80 1.54.The yield of D-mannose isomerase reached 68.28 U/mL under the optimal condition. |
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| Keywords: | isomerase D-mannose optimization Pesudomonas sp |
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