| 新生大鼠海马分区培养神经元对缺氧反应的差异 |
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| 引用本文： | Yao H,Huang YH,Liu ZW,Wan Q,Ding AS,Zhao B,Fan M,Wang FZ. 新生大鼠海马分区培养神经元对缺氧反应的差异[J]. 生理学报, 1998, 50(1): 61-66 |
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| 作者姓名： | Yao H Huang YH Liu ZW Wan Q Ding AS Zhao B Fan M Wang FZ |
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| 作者单位： | 军事医学科学院基础医学研究所神经生物学研究室,北京,100850 |
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| 基金项目： | 国家自然科学基金!No.39570278,中国科学院低氧生理开放实验室经费资助!No.875001 |
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| 摘 要： | 本文以混合培养海马神经元技术为基础,通过改进解剖方法、摸索鼠龄与存活率之间的关系,成功地进行了海马神经凶的分区培养。同时采用同视野跟踪记数法、激光扫描共降焦显微镜测钙和原位杂交等技术,研究了缺氧条件下培养的海马ＣＡ１和ＤＧ神经元存在活率、细胞内游离钙和脑源性神经营养因子（ＢＤＮＦ）ｍＲＮＡ表达水平等指标上变化的差异。结果表明,在相同的缺氧条件下,ＤＧ细胞比ＣＡ１细胞损伤轻微并且具有比ＣＡ１细胞更强
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| 关 键 词： | 海马 缺氧 脑源性 神经营养因子 |
| 修稿时间： | 1997-03-10 |
The different responses to anoxia in cultured CA1 and DG neurons from newborn rats |
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| Yao H,Huang Y H,Liu Z W,Wan Q,Ding A S,Zhao B,Fan M,Wang F Z. The different responses to anoxia in cultured CA1 and DG neurons from newborn rats[J]. Acta Physiologica Sinica, 1998, 50(1): 61-66 |
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| Authors: | Yao H Huang Y H Liu Z W Wan Q Ding A S Zhao B Fan M Wang F Z |
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| Affiliation: | Institute of Basic Medical Sciences, Beijing 100850. |
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| Abstract: | Tissue culture from hippocmpal CA1 or dentate gyrus(DG)region was established on the basis of previous neuronal culture technique.The viability,intracellular calcium concentration and brain-derived neurotrophic factor(BDNF)mRNA expression of the two kinds of neurons after anoxia were observed in the counting in the confocal microscopic field and in situ hybridization.It is found that DG neurons are not only more resistant to anoxia,but also have a stronger ability to keep calcium homeostasis and to express BDNF mRNA than CA1 neurons. |
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| Keywords: | hippocampus anoxia [Ca2 ]i brain-derived neurotrophic factor(BDNF) |
| 本文献已被 维普 万方数据 PubMed 等数据库收录！ |
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