Microbial sensors for determination of aromatics and their chloroderivatives. Part II: Determination of chlorinated phenols using a Rhodococcus-containing biosensor |
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| Authors: | Klaus Riedel Jürgen Hensel Steffen Rothe Barbara Neumann Frieder Scheller |
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| Institution: | (1) Dr. Bruno Lange GmbH, Willstätter Strasse 11, W-4000 Düsseldorf, Federal Republic of Germany;(2) Halle-Wittenberg, Biotechnicum, Martin-Luther-University, Am Kirchtor 1, O-4200 Halle, Federal Republic of Germany;(3) Leuna-Merseburg, Section Biotechnology, Technical High School, Geusaer Str. 3, O-4200 Merseburg, Federal Republic of Germany;(4) Max-Delbrück-Centrum of Molecular Medicine, Robert-Rössle-Str. 10, O-1115 Berlin-Buch, Federal Republic of Germany |
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| Abstract: | An amperometric biosensor for determination of phenol and chlorophenols using Rhodococcus has been developed. This sensor is more sensitive to phenol and chlorophenols, especially to mono- and dichlorinated phenol, than to benzoate and monochlorobenzoates. The incubation of the sensor with phenol and its chlorinated derivatives enhanced the activity of the microbial sensor for these compounds. A linear relationship between the current range and the concentration of phenol, 2-, 3- and 4-chlorophenol was observed up to 20  mol/l. The detection limit for all studied substrates was 4 mol/l. The current difference was reproducible within 5.5% when the test solution contained 40 mol phenol/l.
Correspondence to: K. Riedel |
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