前体对水母雪莲悬浮培养细胞黄酮合成的影响

在水母雪莲（Saussurea medusa Maxim)细胞悬浮体系中加入苯丙氨酸、肉桂酸和乙酸钠3种前体。结果显示，3种前体均能促进细胞内黄酮的生物合成，但它们对细胞的生长也有一定的抑制作用。实验表明，3种前体的添加时间均以第6天为宜。苯丙氨酸的最佳添加浓度为0.05mmol/L，肉桂酸、乙酸钠的最佳添加浓度都是0.1mmol/L。3种前体中，难溶于水的肉桂酸对黄酮合成的促进作用最强，它可使培养物的黄酮产量高达1801mg/L，是对照1．98倍。苯丙氨酸、乙酸钠两种前体协同添加，比它们单独加入更能促进细胞的黄酮合成。

The Effect of Precursor Feeding on Flavonoids Biosynthesis in Cell Suspension Cultures of Saussurea medusa

The effect of three precursors on flavonoids biosynthesis in cell suspension cultures of Saussurea medusa Maxim has been studied. The results show that all these precursors can promote the flavonoids biosynthesis. The optimum time for precursor feeding (OTPF) is the 6th day during the cell culture. L-pheylalanine, cinnamic acid and NaAc have the most significant promoting effect on flavonoids formation at the concentration of 0.1 mmol/L,0.05 mmol/L and 0.1 mmol/L respectively. Of the three precursors, cinnamic acid is the most effective. The maximum flavonoids production reached 1?801 mg/L, 1.98 times as that of the control. When L-pheylalanine and NaAc were combined to be added, they have a more significant promoting effect than they were added respectively.