Direct cleavage of a DNA fragment by a bleomycin-oligonucleotide derivative |
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| Authors: | D S Sergeyev T S Godovikova V F Zarytova |
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| Institution: | Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk. |
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| Abstract: | Bicomycin A1 oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe2+ ions and 2-mercaptoethanol, Blm-R-pd(CCAAACA) (I) damaged the target, pd(TGTTTGGCGAAGGA), with the yield of 80%, without affecting its own oligonucleotide tail. The sites of the cleavage were T?-T? and G?-G?. Unbound bleomycin A5, damaged the G6-G7-G8 site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (ΔTm=11°C) |
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| Keywords: | Reactive oligonucleotide derivative Antitumor antibiotic Bleomycin Direct Damage of nucleic acids |
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