Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B. oleracea var. capitata |
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| Authors: | T Sretenovi?-Raji?i? S Ninkovi? J Milju?-?uki? B Vinterhalter D Vinterhalter |
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| Institution: | (1) Centre for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, Serbia and Montenegro;(2) Institute for Biological Research “S. Stanković”, Bulevar despota Stefana 142, 11000 Belgrade, Serbia and Montenegro;(3) Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11000 Belgrade, Serbia and Montenegro;(4) Present address: Molecular Markers Research Group, IPK Gatersleben, Corrensstr. 3, D-06466 Gatersleben, Germany |
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| Abstract: | Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all
genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes.
In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy
root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive
whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence
of the GUS gene in regenerated shoot clones and in T1 progeny. |
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| Keywords: | cabbage GUS gene hairy roots plant regeneration Savoy cabbage T1 progeny |
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