The mitotic apparatus in the dinoflagellateAmphidinium carterae |
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Authors: | S B Barlow R E Triemer |
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Institution: | (1) Department of Pharmacology, University of Texas Health Science Center-Houston, Houston, Texas;(2) Bureau of Biological Research, Department of Biological Sciences, Rutgers University, Piscataway, New Jersey |
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Abstract: | Summary Aspects of mitosis in the dinoflagellateAmphidinium carterae have been examined using TEM, SEM and fluorescence immunochemistry. The extranuclear spindle is composed of 2–4 bundles of microtubules arranged into two interdigitated half-spindles. Unlike previous reports of dinomitosis, the spindle bundles converge at the poles. These bundles of microtubules are inserted into a multilobed, vesiculate body containing electron opaque, amorphous material. This spindle pole body has ribosomes associated with it and is continuous with the endoplasmic reticulum. Chromosomes are attached to the nuclear envelope, which is persistent throughout mitosis. Kinetochore microtubules attach to the nuclear envelope via elongate electron dense kinetochores (one microtubule per daughter kinetochore). Several microtubules pass alongside the kinetochore, forming a halo of 3–4 spindle microtubules. Electron dense connections can be seen between some of these microtubules and the kinetochore. Chromosome segregation appears to be a function of spindle elongation (anaphase B), since chromosome-to-pole distance (anaphase A) remains relatively unchanged throughout mitosis.Abbreviations DABCO
1,4 diazabicyclo(2,2,2)octane
- EGTA
ethyleneglycol-bis-(-aminoethyl ether)-N,N,N N -tetraacetic acid
- PIPES
piperazine-N,N -bis(2-thanesulfonic acid)
Supported by a Charles and Johanna Bush Predoctoral Fellowship to S. B. B. |
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Keywords: | Amphidinium Spindles Dinoflagellate Mitosis Spindle pole body |
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