第三代测序技术的主要特点及其在病毒基因组研究中的应用 |
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引用本文: | 袁易,王铭杰,张欣欣. 第三代测序技术的主要特点及其在病毒基因组研究中的应用[J]. 微生物与感染, 2016, 0(6): 380-384. DOI: 10.3969/j.issn.1673-6184.2016.06.011 |
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作者姓名: | 袁易 王铭杰 张欣欣 |
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作者单位: | 上海交通大学医学院附属瑞金医院临床病毒研究室 ,上海,200025 |
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基金项目: | 国家"十二五"科技重大专项(2012ZX10002007) |
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摘 要: | 随着基因测序技术的创新和应用,新的高通量测序技术不断涌现,以Pacific Biosciences(PacBio)公司的单分子实时测序(single molecule real time sequencing)为代表的第三代测序(third generation sequencing,TGS)技术开始逐渐应用于基因组研究,包括大型基因组拼装、基因结构变异和表观遗传研究等方面。本文主要对TGS技术的原理、特点和应用,特别是在病毒研究中的应用进行介绍,并与第二代测序(next generation sequencing,NGS)技术进行比较,为基因组测序技术的选择及其临床应用提供一定参考。
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关 键 词: | 第三代测序技术 PacificBiosciences 单分子实时测序 病毒基因组 |
Characteristics of the third generation sequencing technology and its application in researching of viral genomes |
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Abstract: | With the consecutive innovation and application of gene sequencing technology , the third generation sequencing (TGS) based on single molecule real time (SMRT ) sequencing technology has demonstrated a potential in a variety of applications in basic and clinical studies ,including whole genome assembly ,detection of gene structure variation ,epigenetic research ,etc .This review mainly introduces the principle , characteristics and applications of the TGS technology , especially its applications in virus researches .This review also compares the differences between the next generation sequencing (NGS) and TGS ,so as to provide a reference for application based selection for sequencing technology . |
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Keywords: | Third generation sequencing Pacific Biosciences Single molecule real time sequencing Viral genome |
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