Effect of low-dose acute X-irradiation on the frequencies of chromosomal aberrations in human peripheral lymphocytes in vitro |
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| Authors: | J Pohl-Rüling P Fischer O Haas G Obe A T Natarajan P P van Buul K E Buckton N O Bianchi M Larramendy M Kucerová Z Poliková A Leonard L Fabry F Palitti T Sharma W Binder R N Mukherjee U Mukherjee |
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| Institution: | 1. Division of Biophysics, University of Salzburg, Erzabt-Klotz-Strasse 11, A-5020 Salzburg Austria;2. Institute of Applied and Experimental Oncology, University of Vienna, Austria;3. Institute of Genetics, Free University of Berlin, D-1000 Berlin 33 F.R.G.;4. Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden and J.A. Cohen Institute of Radiopathology and Radiation Protection, Leiden The Netherlands;5. MRC, Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburg Scotland;6. IMBICE, La Plata Argentina;7. Genetics Department of the Post-Graduate Medical School, Prague Czechoslovakia;8. Mammalian Genetics Laboratory, Department of Radiobiology, CEN-SCK, B-2400 Mol Belgium;9. Institute of Genetics, University of Rome Italy;10. Department of Zoology, Banares Hindu University, Varanasi 221005 India;11. Institute of Radiotherapy and Radiobiology, University of Vienna Austria;12. International Atomic Energy Agency, Vienna Austria;13. Institute of Biology, Research Centre Seibersdorf, Austria |
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| Abstract: | In a coordinated research programme sponsored by the International Atomic Energy Agency, the frequencies of chromosomal aberrations induced in peripheral blood lymphocytes (in vitro) by 250 kV X-rays at low doses (0.4, 1, 2, 3, 5, 10 and 30 rad) were determined. Blood from 2 donors was used to conduct one master experiment at these dose levels. The culture time used was 48 h and all samples including the controls were processed according to a standard protocol. The coded slides were scored by investigators from 10 participating laboratories. The main results are the following: (1) the frequencies of all types of chromosome aberrations at 0.4 rad are significantly lower than the control values; (2) there is no increase in the frequencies of dicentrics up to 2 rad and in those of terminal deletions up to 5 rad; (3) the mean frequencies of all aberrations considered together are not significantly different from one another at 1, 2 and 3 rad (P = 0.05); and (4) over the entire dose range the dose-effect relationship is clearly non-linear. A fit of these data to a linear quadratic model (E(D) = c + alpha D + beta D2) showed that the observed total aberration frequencies at doses 1, 2, 3 and 5 rad are below the curve defined by the model. The deviations can be explained by an altered kinetics of aberration production at very low doses probably due to DNA repair mechanisms operating these cells. |
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| Keywords: | LSQ least squares method ML maximum likelihood PL peripheral lymphocytes |
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