Identification of copper-zinc superoxide dismutase gene from enteroaggregative Escherichia coli. |
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Authors: | R Kumar M K Sidhu N K Ganguly A Chakraborti |
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Institution: | Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India. |
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Abstract: | We describe here the identification of sodC gene from enteroaggregative Escherichia coli (EAggEC). A 294 bp gene-specific fragment was amplified from the organism by DNA as well as RT-PCR using primers from bacterial sodC sequences. The metal co-factor present in the protein was confirmed by running samples in native gels and inhibiting with 2 mM potassium cyanide. However, the nonpathogenic E. coli possesses the gene but does not express it. Thus, the presence of copper-zinc superoxide dismutase encoded by sodC was demonstrated for the first time in EAggEC, which means it could be a novel candidate for a virulence marker. |
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Keywords: | sodC EAggEC pathogenesis |
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